Goat IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb346969

Description

Goat IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 1

Tested Applications	FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Goat
Application Notes	Mouse Anti-Goat IgG (H&L) Antibody Fluorescein Conjugated has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Mouse
Clonality	Polyclonal
Isotype	IgG
Immunogen	Goat IgG whole molecule
Purity	Goat IgG (H&L) Antibody Fluorescein Conjugated was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse Serum, Goat IgG and Goat Serum. No reaction was observed against Rabbit, Human and Mouse Serum Proteins.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Mouse anti-Goat IgG Antibody Fluorescein Conjugation, Mouse anti-Goat IgG FITC Conjugated Antibody

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Goat IgG (H&L) Antibody Fluorescein Conjugated

Western Blot of Mouse anti-Goat IgG Fluorescein Conjugated Secondary Antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None.

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence