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Description
Research Area
Cell Biology
Images & Validation
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Item 1 of 1
| Tested Applications | FC, FLISA, IF, WB |
|---|---|
| Dilution Range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Goat |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Goat IgG whole molecule |
| Purity | Goat IgG (H&L) Antibody Fluorescein Conjugated was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse Serum, Goat IgG and Goat Serum. No reaction was observed against Rabbit, Human and Mouse Serum Proteins. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mouse anti-Goat IgG Antibody Fluorescein Conjugation, Mouse anti-Goat IgG FITC Conjugated Antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
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