Goat IgG (H&L) Antibody Pre-Adsorbed

SKU: orb347028

Description

Goat IgG (H&L) antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Goat
Application Notes	Anti-Goat IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG
Immunogen	Goat IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.07 mg/mL
Disclaimer	For research use only

Alternative Names

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donkey anti-goat IgG antibody pre-adsorbed

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ELISA Results of Donkey Anti-Goat IgG Antibody (Min X Ch GP Ham Hs Ms Rb Rt Serum proteins) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n orb2652767) as well as Chicken IgG (p/n orb2652774), Guinea Pig IgG (p/n orb2652762), Hamster IgG (p/n orb2652759), Horse IgG (p/n orb2652757), Mouse IgG (p/n orb2652749), Rabbit IgG (p/n orb2652747), and Rat IgG (p/n orb2652744). The working dilution is 1:56000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate (p/n orb348651).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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