Goat Rabbit IgG (H&L), F(ab)'2 fragment, Unconjugated Antibody

SKU: orb700363

Description

Goat polyclonal antibody to Goat anti-Rabbit IgG (H&L)

Images & Validation

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Tested Applications	ELISA
Dilution range	The optimal working dilution should be determined by the investigator
Reactivity	Rabbit
Application Notes	This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Purity is ≥ 90 % based on SDS-PAGE. May contain small amounts of intact IgG

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Purified Rabbit IgG, whole molecule
Purity	Purified goat IgG, F(ab)'2 fragment
Purification	Immunogen affinity purified goat IgG, F(ab)'2 fragment.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store lyophilized material at 2-8°C,For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity, For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol, Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming,
Form/Appearance	Lyophilized
Disclaimer	For research use only

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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