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GP1BA Recombinant Rabbit Monoclonal Antibody
SKU: orb1063827
Featured
Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 9B1 |
| Immunogen | A synthesized peptide derived from human Glycoprotein Ibalpha (1-62/652aa) |
| Target | GP1BA |
| Molecular Weight | 67 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Antigen CD42b alpha; BSS; CD 42b; CD42b alpha; CD42b antigen; GLYCOCALICIN; Glycoprotein Ib(platelet) alpha polypeptide; Glycoprotein Ibalpha; GP Ib alpha; GP1B; CD42b; GPIb alpha; MGC34595; Platelet glycoprotein Ib alpha chain; Platelet glycoprotein Ib alpha polypeptide; Platelet membrane glycoprotein 1b alpha subunit; GP1BA_HUMAN.
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: Human platelets, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 72 kDa, Observed MW: 120 kDa.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD42b) Monoclonal Antibody, Unconjugated (orb1063827) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD42b) Monoclonal Antibody, Unconjugated (orb1063827) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Quick Database Links
Gene Symbol
GP1BA
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
GP1BA Recombinant Rabbit Monoclonal Antibody (orb1063827)
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