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Description
Research Area
Cancer Research, Inflammation, Macrophage
Images & Validation
−Item 1 of 5
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-1000, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BH0F7 |
| Target | GPNMB |
| Molecular Weight | 95,120 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HGFIN; NMB; PLCA3; DC-HIL; Dchil; ipd; GPNMB_HUMAN; GPNMB; Hematopoietic growth factor inducible neurokinin-1 type; GPNMB_MOUSE; Dendritic cell-associated transmembrane protein; Osteoactivin; GPNMB_RAT;
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: A375, 2: B16-F0, 3: EL4.IL-2, 4: HeLa, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 64 kDa, Observed MW: 120, 95, 65 kDa.
Cell line: A375, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1173109.
Cell line: EL4, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1173109.
Tissue: Human tonsil, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173109.
Tissue: Mouse small intestine, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173109.
Quick Database Links
Gene Symbol
GPNMB
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
GPNMB Recombinant Rabbit Monoclonal Antibody (orb1173109)
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