GRF2 Rabbit Polyclonal Antibody

SKU: orb183917

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Description

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GRF2 (20-120/1237aa)
Target	RAPGEF1
Molecular Weight	140 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2(specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor(GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN.

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Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HL-60 cell lysates, Lane 3: Human JAR cell lysates, Lane 4: Human HeLa cell lysates, Primary: Anti-GRF2 (orb183917) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140 kDa, Observed band size: 140 kDa.

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-GRF2 (orb183917) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140 kD, Observed band size: 120 kD.

Quick Database Links

Gene Symbol

RAPGEF1

UniProt

Q13905

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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