Recombinant GH Antibody (pituitary) / Growth Hormone

SKU: orb2642651

Description

Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly).

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant GH antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rGH/1450
Immunogen	A human partial recombinant protein corresponding to amino acids 58-187 was used as the immunogen for this recombinant GH antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant GH Antibody (pituitary) / Growth Hormone

IHC testing of FFPE human pituitary gland with recombinant GH antibody (clone rGH/1450). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant GH antibody (clone rGH/1450) as confirmation of integrity and purity.

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UniProt

P01241

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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