Recombinant GRP94 Antibody / HSP90B1

SKU: orb639975

Description

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Research Area

Epigenetics

Images & Validation

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Tested Applications	FACS, IHC-P
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant GRP94 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	HSP90B1/3168R
Immunogen	A recombinant full-length human protein was used as the immunogen for this recombinant GRP94 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Flow cytometry testing of PFA-fixed human HepG2 cells with recombinant GRP94 antibody (clone HSP90B1/3168R); Red=isotype control, Blue= recombinant GRP94 antibody.

IHC staining of FFPE breast carcinoma with recombinant GRP94 antibody (clone HSP90B1/3168R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant GRP94 antibody (clone HSP90B1/3168R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P14625

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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