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Featured
KO/KD
Validated
Validated
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IP, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3B (NP_001139628.1). |
| Target | GSK3B |
| Molecular Weight | Observed: 46kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta isoform, TPKI, GSK 3beta, Tau protein kinase I, ZGSK-3beta
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Western blot analysis of extracts of various cell lines, using GSK3B antibody (orb1274437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (orb1274437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using GSK3B antibody (orb1274437) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug GSK3B antibody (orb1274437). Western blot was performed from the immunoprecipitate using GSK3B antibody (orb1274437) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
GSK3B
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
GSK3B Antibody (orb1274437)
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