Guinea pig Immunoglobulin M (IgM) ELISA Kit

SKU: orb2668162

Description

Images & Validation

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Application Notes

standard: 200 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea Pig IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea Pig IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea Pig IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea Pig IgM in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 µg/mL
Sensitivity	1.35 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

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