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Description
Images & Validation
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| Tested Applications | WB |
|---|---|
| Dilution Range | WB: 1:1000, WB: 1:1000, WB: 1:1000 |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | KLH conjugated synthetic peptide encoding HA tag was used as antigen. |
| Target | KLH conjugated synthetic peptide encoding HA tag was used as antigen. |
| Molecular Weight | 45-50 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Glutathione S-transferase
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All lanes: Anti-HA Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 12tag lysate (10ug). Lane 2: Non-transfected 293T/17 lysate (10ug). Lane 3: 12tag recombinant protein lysate (0. 1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-HA Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 12tag lysate (10ug). Lane 2: Non-transfected 293T/17 lysate (10ug). Lane 3: 12tag recombinant protein lysate (0. 1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-HA Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 12tag lysate (0. 5ug). Lane 2: Non-transfected 293T/17 lysate (0. 5ug). Lane 3: 12tag recombinant protein lysate (0. 1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
KLH conjugated synthetic peptide encoding HA tag was used as antigen.
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HA Tag Antibody (orb1787856)
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