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HAVCR1 Antibody
SKU: orb1240149
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Description
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-TIM-1 antibody (orb1240149) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1. |
| Target | HAVCR1 |
| Molecular Weight | Predicted: 44kD Observed: 60kD (Post-modification: 4 N-linked glycosylation) |
| Purification | TIM-1 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1
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![Anti-Tim-1 [3B3]](/images/pub/media/catalog/product/NewWebsite/35/orb613962_1.png)
![Anti-Tim-1 [3B3]](/images/pub/media/catalog/product/NewWebsite/35/orb613962_2.png)
![Anti-Tim-1 [3B3]](/images/pub/media/catalog/product/NewWebsite/35/orb613963_1.png)
![Anti-Tim-1 [3B3]](/images/pub/media/catalog/product/NewWebsite/35/orb613963_2.png)
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Western Blot Validation in Human Cell Lines. Loading: 15 μg of lysates per lane. Antibodies: TIM-1 orb1240149 (8 μg/mL), overnight incubation at 4°C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 μg of lysates per lane. Antibodies: TIM-1 orb1240149 (8 μg/mL), TIM-1 orb1238784 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4°C (orb1240149) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Validation with TIM-1 siRNA Knockdown in Hela Cells. HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2). Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: orb1240149 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 μg of lysates. Antibodies: TIM-1 orb1240149, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of TIM-1 in Human Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling TIM-1 with orb1240149 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunofluorescence Validation of TIM-1 in Mouse Kidney. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney tissue labeling TIM-1 with orb1240149 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HAVCR1 Antibody (orb1240149)
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