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HCMV pp65 Rabbit Polyclonal Antibody
SKU: orb10511
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Description
Images & Validation
−Item 1 of 1
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Virus |
| Predicted Reactivity | Virus |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from HCMV pp65 (251-350/561aa) |
| Target | UL16 |
| Molecular Weight | 65 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−PP65_HCMVM; PP65_HCMVA; Cytomegalovirus pp65; 65 kDa lower matrix phosphoprotein; 65 kDa matrix phosphoprotein; 65 kDa phosphoprotein; CMV 65 kDa lower matrix phosphoprotein; CMV pp65; Cytomegalovirus 65 kDa lower matrix phosphoprotein; Cytomegalovirus pp65; HHV 5; PP65; Tegument protein pp65; Tegument protein UL83; UL83; HCMV PP65.
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cytomegalovirus pp65 Polyclonal Antibody, Unconjugated (orb10511) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
UL16
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Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Liu, Xiaoyang et al. Human cytomegalovirus evades antibody-mediated immunity through endoplasmic reticulum-associated degradation of the FcRn receptor Nat Commun, 10, 3020 (2019)
HCMV pp65 Rabbit Polyclonal Antibody (orb10511)
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