HCN4 Rabbit Polyclonal Antibody

SKU: orb10785

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Description

Research Area

CARdiovascular Research, Cell Biology, Channel Protein, Gustatory System, Ion Channel

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human HCN4 (501-600/1203aa)
Target	HCN4
Molecular Weight	129 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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HCN 4; Hyperpolarization activated cyclic nucleotide gated potassium channel 4; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4; Hyperpolarization activated Cyclic Nucleotide-gated channel 4; HCN4_HUMAN.

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Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-HCN4 antibody (orb10785), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-HCN4 (orb10785) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 129 kD, Observed band size: 129 kD.

Sample: Lane 1: Mouse Testis Lysates, Lane 2: Rat Testis Lysates, Lane 3: Human HL-60 cell Lysates, Lane 4: Human MCF-7 cell Lysates, Lane 5: Human A549 cell Lysates, Primary: Anti-HCN4 (orb10785) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 129kDa, Observed band size: 129kDa.

Quick Database Links

Gene Symbol

HCN4

UniProt

Q9Y3Q4

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence