Helicobacter pylori Antibody (Catalase) / H. pylori

SKU: orb1822653

Description

The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	H. pylori
Application Notes	Optimal dilution of the Helicobacter pylori antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	HPYL/8548R
Immunogen	A recombinant Helicobacter pylori Catalase protein fragment (within amino acids 323-445) was used as the immunogen for the Helicobacter pylori antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Helicobacter pylori Antibody (Catalase) / H. pylori

SDS-PAGE analysis of purified, BSA-free Helicobacter pylori antibody (clone HPYL/8548R) as confirmation of integrity and purity.

IHC staining of FFPE human H. pylori-infected stomach tissue with Helicobacter pylori antibody (clone HPYL/8548R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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