HES1 Antibody (N-term T24)

SKU: orb36445

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Description

Purified rabbit polyclonal antibody.

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, WB
Dilution range	WB: 1:1000, IF/ICC: 1:10-50, FACS: 1:10-50
Reactivity	Human
Predicted Reactivity	Mouse, Other, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB13980
Immunogen	Synthetic Peptide
Target	HES1
Molecular Weight	29541
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Anti-Transcription factor HES-1 antibody, anti-Hairy and enhancer of split 1 antibody, anti-Hairy-like antibody, anti-HHL antibody, anti-Hairy homolog antibody, anti-HL antibody, anti-HRY antibody

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Western blot analysis of anti-HES1 Antibody (N-term T24) in HL60 cell line lysates (35 ug/lane). HES1 (arrow) was detected using the purified Pab.

HES1 Antibody (N-term T24) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HES1 Antibody (N-term T24) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

HES1

UniProt

Q14469

RefSeq (Protein):NP_005515.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005515.1

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence