HIF-1 Alpha Recombinant Rabbit Monoclonal Antibody

SKU: orb2561577

Description

Research Area

Epigenetics and Nuclear Signaling, Jumonji Containing Proteins, Signal Transduction, Transcription Factors

Images & Validation

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Item 1 of 1

Tested Applications	ICC, WB
Dilution Range	WB=1:500-2000, ICC/IF=1:50-200
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Immunogen	A synthesized peptide derived from human HIF 1 alpha (300-550/826aa)
Target	HIF1A
Molecular Weight	110 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A.

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HIF-1 Alpha Recombinant Rabbit Monoclonal Antibody

Western blot analysis of HeLa treated with CoCl2 cell lysate. Using HIF-1 Alpha (orb2561577) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Quick Database Links

Gene Symbol

HIF1A

UniProt

Q16665

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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