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His Tag (2A2) Mouse mAb
SKU: orb1562825
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IF, IP, WB |
|---|---|
| Dilution range | WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/200 |
| Reactivity | Other |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone No. | 2A2-4H2-10D8 |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH. |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
2ug His fusion protein+ Primary antibody dilution at 1) 1:5000. 2) 1:10000.
Immunofluorescence analysis of His Tag (2A2) in 293 cells transfected with a Histag protein using His Tag (2A2) antibody (red), and DAPI (blue).
Immunoprecipitation analysis of Flag Tag (7E1) in 1: untransfected 293 cell lysates 2: transfected 293 cell lysates with Histag fusion protein 3: IP (untransfected 293+antiHis mAb+ Protein G agarose) 4: IP (transfected 293+ normal mouse IgG+Protein G agarose) 5: IP (transfected 293+antiHis mAb+ Protein G agarose).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
His Tag (2A2) Mouse mAb (orb1562825)
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