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H1F0 Antibody

SKU: orb1252534

Description

H1F0 Antibody

Images & Validation

Tested ApplicationsFC, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
Application Notes
Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone No.1415-1
ImmunogenNuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
TargetH1F0
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
DisclaimerFor research use only

Alternative Names

H1F0, H10, H1FV, MGC5241

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H1F0 Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).

H1F0 Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).

H1F0 Antibody

FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1).

H1F0 Antibody

FFPE human pancreas tested with Histone H1 antibody (1415-1).

H1F0 Antibody

FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1).

H1F0 Antibody

FFPE human angiosarcoma tested with Histone H1 antibody (1415-1).

H1F0 Antibody

Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

H1F0 Antibody (orb1252534)

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