Histone Antibody / Histone H1

SKU: orb317050

Description

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. View the recombinant form of this Histone H1 antibody.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	1415-1
Immunogen	Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone H1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1)

IHC: FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1)

IHC: FFPE human pancreas tested with Histone H1 antibody (1415-1)

IHC: FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1)

IHC: FFPE human angiosarcoma tested with Histone H1 antibody (1415-1)

Western blot testing of human heart lysate with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).

Immunofluorescent staining of fixed human HeLa cells with Histone antibody (clone 1415-1, green) and Phalloidin (red).

Flow cytometry testing of fixed human HeLa cells with Histone antibody (clone 1415-1); Red = isotype control, Blue = Histone antibody.

SDS-PAGE analysis of purified, BSA-free Histone antibody (clone 1415-1) as confirmation of integrity and purity.

Quick Database Links

UniProt

P07305

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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