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H2AFX Antibody
SKU: orb1255416
Featured
Description
Images & Validation
−Item 1 of 10
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human Histone H2AX (NP_002096.1). |
| Target | H2AFX |
| Molecular Weight | Observed: 16kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2AFX, H2A.X, H2A/X, H2AX
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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (orb1255416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2AX antibody (orb1255416) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody (orb1261278) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
H2AFX
UniProt
UniProt Details
− No UniProt data available
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Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
H2AFX Antibody (orb1255416)
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