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H3C1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution range | WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200 IP: 1/20 |
| Reactivity | Human, Mouse, Rat, Yeast |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone No. | 6G2-5A6-6C1 |
| Immunogen | Recombinant Protein of Histone H3 |
| Target | H3C1 |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
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Western blot analysis of Histone H3 (6G2) in C6, 3T3 and MCF-7 lysates using Histon H3 antibody.

Immunofluorescence analysis of Histone H3 in Hela using Histone H3 antibody (Right) and DAPI (Left).

Immunoprecipitation analysis of Histone H3 (6G2) in Hela lysates using Histone H3 (6G2) antibody.

Immunohistochemistry analysis of paraffin-embedded rat testis tissue using Histone H3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Histone H3 in C6, 3T3, Hela lysates using Histone H3 (6G2) antibody.
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Protocol Information
H3C1 Antibody (orb1566578)
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