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Description
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Dilution Range | WB: 1:1000, WB: 1:3000 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | This Histone H3 antibody is generated from a mouse immunized with Histone H3 recombinant protein. Antigen Region: 1-156 aa. |
| Target | H3C1 (HGNC:4766) |
| Molecular Weight | 15404 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA
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Western blot analysis of lysates from rat C6, Hela cell line (from left to right), using Histone H3 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
All lanes: Anti-Histone H3 Antibody at 1: 3000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: C2C12 whole cell lysate. Lane 7: PC-12 whole cell lysate. Lane 8: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
H3C1 (HGNC:4766)
UniProt
UniProt Details
− No UniProt data available
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Histone H3 Antibody (orb1788202)
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