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Histone H3 K27ac Antibody
SKU: orb420433
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, ELISA, IP, WB |
|---|---|
| Dilution Range | ELISA: 1: 10,000, IP: 1:100, WB: 1:500-1:1000 |
| Reactivity | C. elegans, Human, Monkey, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Histone H3 [ac Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27ac site of human Histone H3. |
| Purity | Anti-Histone H3 [ac Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [ac Lys27] from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.37 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Histone H3 Ac Lys27 antibody, Histone H3 (K27 modification), H3 K27-Ac, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K27ac
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Dot Blot of Rabbit Histone H3 [ac Lys27] Antibody. Lane 1: K27 unmodified. Lane 2: K27-Ac. Lane 3: K27-Me1. Lane 4: K27-Me2. Lane 5: K27-Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [ac Lys27] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
Western Blot of Histone H3 [ac Lys27] (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 2: HeLa cell lysates. Lane 3: NIH/3T3 cell lysates. Lane 4: Cos 7 cell lysates. Lane 5: C. Elegans lysates. Load: 35 µg per lane. Primary antibody: Histone H3 [ac Lys27] antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 [ac Lys27]. Other band(s): non-specific.
Quick Database Links
UniProt
RefSeq (Protein):NP_001005464.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001005464.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
DOT
Dot Blot
Histone H3 K27ac Antibody (orb420433)
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