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Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody

SKU: orb1172850

Description

Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsChIP, WB
Dilution rangeWB=1:500-2000, ChIP=6 ug/5x106 cells
ReactivityHuman, Mouse, Rat
Predicted ReactivityRat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
TargetH3C1
PurificationAntigen affinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only
Quality Guarantee

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, N2a, BRL, Mouse kidney, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: N2a, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ChIP
Chromatin Immunoprecipitation
View Protocol

Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody (orb1172850)

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50 μl
$ 320.00
100 μl
$ 460.00
DispatchUsually dispatched within 5-10 working days
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