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Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | ChIP, WB |
|---|---|
| Dilution range | WB=1:500-2000, ChIP=6 ug/5x106 cells |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target | H3C1 |
| Purification | Antigen affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |

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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, N2a, BRL, Mouse kidney, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: N2a, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.
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Histone H3 (tri methyl K4, serotonyl Q5) Rabbit Polyclonal Antibody (orb1172850)
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