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Histone H4 (phospho Ser47) rabbit pAb Antibody
SKU: orb767568
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Description
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−Item 1 of 3
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H4 around the phosphorylation site of Ser47. AA range:13-62 |
| Molecular Weight | 13 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-HIST1H4A antibody, anti-H4/A antibody, anti-H4FA antibody, anti-HIST1H4B antibody, anti-H4/I antibody, anti-H4FI antibody, anti-HIST1H4C antibody, anti-H4/G antibody, anti-H4FG antibody, anti-HIST1H4D antibody, anti-H4/B antibody, anti-H4FB antibody, anti-HIST1H4E antibody, anti-H4/J antibody, anti-H4FJ antibody, anti-HIST1H4F antibody, anti-H4/C antibody, anti-H4FC antibody, anti-HIST1H4H antibody, anti-H4/H antibody, anti-H4FH antibody, anti-HIST1H4I antibody, anti-H4/M antibody, anti-H4FM antibody, anti-HIST1H4J antibody, anti-H4/E antibody, anti-H4FE antibody, anti-HI antibody
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Immunofluorescence analysis of HUVEC cells, using Histone H4 (Phospho-Ser47) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Histone H4 (phospho Ser47) rabbit pAb Antibody (orb767568)
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