Histone H4 (tri methyl K20) Recombinant Rabbit Monoclonal Antibody

SKU: orb559094

Description

Research Area

DNA and RNA, Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB=1:500-1000
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	2C12
Immunogen	KLH conjugated Synthesised methylpeptide derived from human Histone H4 around the methylation site of tri methyl Lys20 HR(tri methyl K)VL
Target	H4C1
Molecular Weight	11 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H4K20me3; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.Histone H3/l.

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Histone H4 (tri methyl K20) Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab, dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, Jurkat, BRL, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Quick Database Links

Gene Symbol

H4C1

UniProt

P62805

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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