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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P-Leica - 1:500 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase). Antigen Region: 78-108 aa. |
| Target | HK1 (HGNC:4922) |
| Molecular Weight | 102486 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1
Quality Guarantee
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HK1 Antibody (L93) western blot analysis in MCF-7 cell line lysates (35 ug/lane).This demonstrates the HK1 antibody detected the HK1 protein (arrow).
Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2).
All lanes: Anti-hHK1-L93 at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes: Anti-HK1 (Hexokinase) Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
HK1 (HGNC:4922)
UniProt
RefSeq (Protein):NP_000179.2, NP_277032.1, NP_277035.2, NP_277033.1, NP_277031.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000179.2, NP_277032.1, NP_277035.2, NP_277033.1, NP_277031.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
HK1 (Hexokinase) Antibody (N-term) (orb1928566)
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