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HLA-ABC Antibody (MHC I)
SKU: orb2638534
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FACS, IF, WB |
|---|---|
| Dilution Range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | 246-B8.E7 |
| Immunogen | Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41 kDa.
SDS-PAGE Analysis of Purified, BSA-Free HLA-ABC Antibody (clone 246-B8.E7). Confirmation of Integrity and Purity of the Antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
HLA-ABC Antibody (MHC I) (orb2638534)
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