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KO/KD
Validated
Validated
Description
Images & Validation
−
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB=1:1000-5000, IHC-P=1:200-1000, IF=1:200-1000, Flow-Cyt=1μg/Test, IHC-F=1:200-1000, ICC/IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B5C8 |
| Immunogen | A synthesized peptide derived from human HMGB1 (150-200aa) |
| Target | HMGB1 |
| Molecular Weight | 27 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HMG-1; HMG1; HMG3; SBP-1; p30; Ac2-008; HMGB1_BOVIN; HMGB1; High mobility group protein 1 (HMG-1); HMGB1_HUMAN; high mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; Sulfoglucuronyl carbohydrate binding protein; Amphoterin; high mobility group protein 1
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Quick Database Links
Gene Symbol
HMGB1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days