Hoechst 33258 trihydrochloride

SKU: orb1303223

Description

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Key Properties

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CAS Number	23491-45-4
MW	533.9
Purity	96.31%
Formula	C₂₅H₂₄N₆O·3HCl
SMILES	CN1CCN(c(cc2)cc3c2nc(c4cc5c(N/C(N5)=C6C=CC(C=C\6)=O)cc4)[nH]3)CC1.Cl.Cl.Cl
Target	Parasite
Solubility	DMSO:6.58 mg/mL (12.32 mM)

Bioactivity

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Target IC50

U-937 cells:15.4 ± .16 μM IC50 |HeLa cells:51.3 ± .56 μM IC50 |HL-60 cells:32.4 ± .27 μM IC50

Cell Research

Solution preparation a. Preparation of stock solution: Dissolve 10 mg in 5 mL DMSO; (It is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) b. Preparation of Hoechst working solution Dilut the stock solution and dissolve it in serum-free cell culture medium or PBS to obtain a Hoechst working solution with a final concentration of 10 μg/ml; (Selec the appropriate working solution concentration according to Experimental requirements and prepare it for immediate use) c. Cell staining 1. Suspended cells (6-well plate) a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discar the supernatant. Wash twice with PBS for 5 minutes Each the cell density is × 06/mL; b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes; c. Centrifuge at 400 g for 3-4 minutes at 4℃, and discar the supernatant; d. Wash twice with PBS, 5 minutes Each time; e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer. 2. Adherent cells a. Culture adherent cells on sterile coverslips; b. Remove coverslips from culture medium and aspirate excess culture medium; c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 3-10 minutes; d. Wash twice with culture medium, 5 minutes Each time. Observe with fluorescence microscope or flow cytometer.

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Disclaimer	For research use only

Alternative Names

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H 33258, H 33258 trihydrochloride, H 33258 Trihydrochloride, H33258, H-33258, H33258 Trihydrochloride, H-33258 Trihydrochloride, Hoechst 33258, HOE 33258, Hoechst 33258 trihydrochloride, Hoechst 33258 Trihydrochloride, HOE33258, HOE-33258, inhibit, Inhibitor, Parasite, Bisbenzimide, bisBenzimide H 33258, bisBenzimide H 33258 Trihydrochloride, bisBenzimide H 33258 trihydrochloride, bisBenzimide H33258, bisBenzimide H-33258, bisBenzimide H33258 Trihydrochloride, bisBenzimide H-33258 Trihydrochloride, Pibenzimol

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Chemical structure of Hoechst 33258 trihydrochloride

Compound Identifiers

PubChem CID

31953 ↗

Key Properties

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No computed properties available.

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