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Hoechst 33342 Staining Kit
SKU: orb219886
Description
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−Storage & Handling
−| Storage | Store at -20°C for one year. |
|---|---|
| Disclaimer | For research use only |
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Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).
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Protocol Information
IF
Immunofluorescence
ICC
Immunocytochemistry
Hoechst 33342 Staining Kit (orb219886)
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