Horse Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU: orb781471

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Atrial Natriuretic Peptide(ANP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Atrial Natriuretic Peptide(ANP).

Research Area

Cardiovascular Research, Metabolism Research

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse ANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ANP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Equine
Sample Types	Serum, plasma, other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	31.25-2000 pg/mL
Sensitivity	9.06 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ANH ELISA Kit, ANF ELISA Kit, CDD ELISA Kit, Atrial Natriuretic Factor ELISA Kit, Atrial Natriuretic Hormone ELISA Kit, Atriopeptin ELISA Kit, Cardionatrine ELISA Kit, Cardiodilatin ELISA Kit

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Horse Atrial Natriuretic Peptide (ANP) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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