Horse Immunoglobulin E (IgE) ELISA Kit

SKU: orb1291281

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Description

Horse IgE (Immunoglobulin E) ELISA Kit

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IgE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Equine
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ug/mL
Sensitivity	0.65 ug/mL
Concentration	100 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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IGHE, Immunoglobulin Heavy Constant Epsilon, Ig epsilon chain C region

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Equine (1)

Sae Yoshida 1, Akira Matsuda 1, Eri Iwata 1, Tetsushi Ono 2, Keiichi Hisaeda 1, Emi Ohzawa 3, Yasuharu Hiasa 4, Hitoshi Kitagawa Allergen-specific immunoglobulin-Es for dermatitis in the Japanese native Noma horses J Vet Med Sci, (2024)

PubMed: 39010246

Applications

ELISA

Reactivity

Equine