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HSF1A

SKU: orb1303135

Description

HSF1A is a cell-permeable small molecule activator of the heat shock factor 1 (HSF1) pathway. It is used in research to investigate cellular stress responses, offering protection against apoptosis in both in vitro cell cultures and in vivo models of proteotoxic stress.

Research Area

Metabolism Research, Signal Transduction

Images & Validation

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HSF1A

Key Properties

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CAS Number1196723-93-9
MW409.52
Purity98.89%
FormulaC21H19N3O2S2
SMILESCCc1ccc(cc1)S(=O)(=O)Nc1cc(nn1-c1ccccc1)-c1cccs1
TargetHSP
SolubilityDMSO:50 mg/mL (122.09 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.88 mM)

Bioactivity

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In Vivo
HSF1A enhances HSF1 activity, stabilizes its expression, and mitigates Doxorubicin (DOX)-induced cardiac damage. In WKY rats treated with DOX (30 mg/kgw) and a combination of DOX and HSF1A (100 mg/kgw/day), HSF1A supplementation significantly restores cardiac function to control levels. Moreover, HSF1A promotes HSF1 nuclear translocation, increases protein chaperone expression, and reduces protein misfolding and cell death in neurodegenerative disease models. Echocardiographic data show that HSF1A also improves cardiac function in the face of DOX-induced impairments.
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In Vitro
HSF1A is a small-molecule activator that enhances HSF1's ability to protect cells from stress-induced apoptosis by binding to and inhibiting TRiC subunits' activity, crucially without disturbing ATP hydrolysis. When the TRiC complex is genetically inactivated or depleted, HSF1 activation occurs in humans, and HSF1A is found to block the direct interaction between purified TRiC and HSF1 in vitro. Fluorescence anisotropy experiments with FITC-tagged HSF1A demonstrate its strong binding to the Tcp1 subunit of TRiC, with an affinity around 600 nM. This interaction is further supported by titration experiments with purified Tcp1, indicating qualitative validation. Moreover, a significant reduction in the number of cells displaying aggregates is observed at HSF1A concentrations as low as 2 μM, with this fraction consistently declining in a dose-dependent manner. Specifically, pretreatment with 12 μM HSF1A leads to approximately 20% of cells showing aggregates under fluorescence microscopy, highlighting HSF1A's potential in reducing cellular stress markers.
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Cell Research
PC12 cells seeded into a 96-well plate (5×104 cells/well) are treated with increasing concentrations of HSF1A (2, 4, 8 and 12 μM) for 15 h, at which time httQ74-GFP expression is stimulated by incubation in the presence of 1 μg/mL Doxycycline for 5 d. Cell viability is assessed via the XTT viability assay.
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Animal Research
RatTen-week-old Wistar Kyoto rats (WKY) are used. The rats are housed at a constant temperature (22°C) on a 12-h light/dark cycle with food and tap water. The animals are arranged into three groups: WKY rats (the control group), DOX rats and DOX rats treated with HSF1A. Each group contain five animals. The DOX group is injected with DOX (5 mg/kg) for 6 consecutive weeks intraperitoneal injection to achieve a cumulative dose of 30 mg/kg, which has been well documented to achieve cardiotoxicity. The small molecular HSF1 activator HSF1A (100 mg/kg/day) is injected intraperitoneally.
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Storage & Handling

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StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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inhibit, Inhibitor, HSP, HSF1A, HSF-1A, HSF1, Heat shock proteins

Similar Products

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  • HSF1A

    HSF1A [orb1226824]

    >98% (HPLC)

    1196723-93-9

    409.522

    C21H19N3O2S2

    2 mg, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg, 1 g, 500 mg, 200 mg
Quality Guarantee

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
44472508 ↗

Key Properties

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No computed properties available.

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HSF1A (orb1303135)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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1 mg
$ 90.00
2 mg
$ 130.00
1 ml x 10 mM (in DMSO)
$ 180.00
5 mg
$ 190.00
10 mg
$ 260.00
25 mg
$ 530.00
50 mg
$ 760.00
100 mg
$ 1,030.00
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