HSP110 Antibody (Biotin)

SKU: orb152134

Description

Rabbit polyclonal to Hsp110 (Biotin). Hsp110 belongs to a family of large stress proteins known as the Hsp110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain. Hsp110 cooperates with Hsp70 in protein folding in the eukaryotic cytosol. In mammals, Hsp110 is constitutively expressed, but exhibits particularly high levels in the brain. Both Hsp70 and Hsp110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of Hsp110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of Hsp70. Studies also suggest that Hsp110 can be used in heat shock protein-based cancer immunotherapy..

Images & Validation

−

Item 1 of 2

Tested Applications	ICC, IF, IHC, WB
Dilution range	WB (1:1000), IHC (1:100)
Reactivity	Bovine, Fish, Hamster, Human, Monkey, Mouse, Rat, Sheep, Yeast
Application Notes	1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
Target	HSP110
Molecular Weight	110kDa
Purification	Protein A Purified
Conjugation	Biotin

Storage & Handling

−

Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

−

HSPH1, HSP110, HSP105, Heat shock protein 105 kDa, Heat shock 110 kDa protein, KIAA0201, Heat shock 105kDa alpha, heat shock 105kDa beta, HSP105A, HSP105B, NY CO 25

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.

Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP110

UniProt

Q92599

NCBI Gene ID

10808

RefSeq (Protein):NP_006635.3

UniProt Details

−

No UniProt data available

NCBI Gene Details

−

No NCBI Gene data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006635.3

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunofluorescence

View Protocol

ICC

Immunocytochemistry

View Protocol

Find More Protocols