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HSP27 Antibody (APC)

SKU: orb147516

Description

Mouse monoclonal to Hsp27 (APC). Hsp27s belong to an abundant and ubiquitous family of Hsp27s belong to an abundant and ubiquitous family of...

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

Tested ApplicationsELISA, FC, ICC, IF, IHC, IP, WB
Dilution RangeWB (1:2000), ICC/IF (1:100)
ReactivityHuman
Application Notes
0.5 µg/ml was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b Kappa
Clone No.5D12-A12
ImmunogenFull length human HSP27
TargetHSP27
Molecular Weight27kDa
PurificationProtein G Purified
ConjugationAPC

Storage & Handling

StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

HSPB1, HSP27, HSP25, HSP28, Heat shock protein beta-1, Heat shock 27 kDa protein, MKBP, DMPK-binding protein, SRP27, 28kDa heat shock protein, CMT2F

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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200 μg
$ 650.00
Free Secondary Antibody (20 ul)0/0

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