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Description
Research Area
Signal Transduction
Images & Validation
−
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000; IHC: 1/50-1/100; IF/ICC: 1/100-1/200 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Peptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27. |
| Target | Disease |
| Molecular Weight | 27kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; HSP 27; HSP25
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence