HSP40 rabbit pAb Antibody

SKU: orb765458

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Description

Rabbit polyclonal antibody to HSP40

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320
Molecular Weight	45
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-DNAJB1 antibody, anti-DNAJ1 antibody, anti-HDJ1 antibody, anti-HSPF1 antibody, anti-DnaJ homolog subfamily B member 1 antibody, anti-DnaJ protein homolog 1 antibody, anti-Heat shock 40 kDa protein 1 antibody, anti-HSP40 antibody, anti-Heat shock protein 40 antibody, anti-Human DnaJ protein 1 antibody, anti-hDj-1 antibody

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Immunofluorescence analysis of Hela cell. 1, HSP40 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 Monoclonal Antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using HSP40 Polyclonal Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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