Recombinant HSP60 Antibody

SKU: orb1151571

Description

Mouse monoclonal antibody to HSP60

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2a, kappa
Clone No.	rHSPD1/6495
Immunogen	Recombinant full-length human HSPD1 protein was used as the immunogen for the recombinant HSP60 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6495). Predicted molecular weight: ~60 kDa.

IHC staining of FFPE human thyroid tissue with recombinant HSP60 antibody (clone rHSPD1/6495). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone rHSPD1/6495) as confirmation of integrity and purity.

Quick Database Links

UniProt

P10809

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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