HSP60 Antibody / HSPD1

SKU: orb248380

Description

This antibody recognizes a 60 kDa protein, identified as the heat shock protein 60. The antibody's epitope is localized between amino acids 383-447 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK1 antibody, unlike LK2 antibody, recognizes only the mammalian (not bacterial) HSP60 and is useful in distinguishing HSP60 from mammals and bacteria.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human, Mouse, Rat
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	LK1
Immunogen	Recombinant human HSP60 was used as the immunogen for this antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human breast carcinoma with HSP60 antibody (clone LK1).

IHC testing of FFPE pancreas tissue with HSP60 antibody (clone LK1).

Western blot testing of HeLa cell lysate with HSP60 antibody (clone LK1).

SDS-PAGE Analysis of Purified, BSA-Free HSP60 Antibody (clone LK1). Confirmation of Integrity and Purity of the Antibody.

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP60 antibody (green, clone LK1) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human HeLa cells with HSP60 antibody (clone LK1); Red = isotype control, Blue = HSP60 antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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