Cart summary

You have no items in your shopping cart.

HSP70 Antibody (PerCP)

SKU: orb146739
FeaturedFeatured Product

Description

Mouse monoclonal to Hsp70 (PerCP). Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Read more...

Images & Validation

−
Tested ApplicationsAM, Blocking, ELISA, FACS, FC, ICC, IEM, IF, IHC, WB
Dilution rangeWB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000)
ReactivityBovine, Canine, Drosophila, Fish, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Sheep
Application Notes
1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

−
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.C92F3A-5
ImmunogenHuman HSP70
TargetHSP70
Molecular Weight70kDa
PurificationProtein G Purified
ConjugationPerCP

Storage & Handling

−
StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

−
HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B

Similar Products

−
    1. HSP70 Antibody (PerCP)
    Item 1 of 7

    HSP70 Antibody (PerCP) [orb151245]

    ELISA,  FC,  ICC,  IF,  IHC,  IP,  WB

    Bovine, Canine, Fish, Guinea pig, Hamster, Human, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rat, Sheep

    Rabbit

    Polyclonal

    PerCP

    100 μg
    1. HSP70/HSC70 Antibody (PerCP)
    Item 1 of 6

    HSP70/HSC70 Antibody (PerCP) [orb146790]

    ICC,  IF,  IHC,  IP,  WB

    Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Yeast

    Mouse

    Monoclonal

    PerCP

    200 μg
    1. HSP70 Antibody (PerCP)
    Item 1 of 6

    HSP70 Antibody (PerCP) [orb54782]

    FACS,  FC,  ICC,  IF,  WB

    Bovine, Human, Mouse, Porcine, Rat

    Mouse

    Monoclonal

    PerCP

    100 μg
    1. HSC70 (HSP73) Antibody (PerCP)
    Item 1 of 4

    HSC70 (HSP73) Antibody (PerCP) [orb147351]

    AM,  ELISA,  ICC,  IF,  IHC,  IP,  PBA,  PLA,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    PerCP

    200 μg
    1. HSP27 Antibody (PerCP)
    Item 1 of 4

    HSP27 Antibody (PerCP) [orb151296]

    FACS,  FC,  ICC,  IF,  IHC,  WB

    Canine, Fish, Human, Mouse

    Rabbit

    Polyclonal

    PerCP

    200 μg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

HSP70 Antibody (PerCP)

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

HSP70 Antibody (PerCP)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse.

HSP70 Antibody (PerCP)

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

HSP70 Antibody (PerCP)

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5. Tissue: Heat Shocked CD3+ CD8+ T cells. Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody at 1:1000.

HSP70 Antibody (PerCP)

Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol
HSP70
UniProt
P0DMV9, P0DMV8
NCBI Gene ID
3303
RefSeq (Protein):NP_005336.3

UniProt Details

−
No UniProt data available

NCBI Gene Details

−
No NCBI Gene data available

NCBI Reference Sequences

−
Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
ProteinNP_005336.3

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
Find More Protocols

HSP70 Antibody (PerCP) (orb146739)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Login to review

Available Sizes

Select a size below

200 μg
$ 740.00
DispatchUsually dispatched within 2-3 weeks
Bulk Enquiry
Need technical support? Find your Distributor Return and Tax Policy

Email

info@biorbyt.com

UK Office

+44 (0)1223 859353

US Office

+1 (415) 906-5211

CN Office

+86 (27)-87663101

Distributors

biorbyt.com/distributor