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HSP70 ELISA Kit (High-Sensitivity)

SKU: orb1822522
FeaturedFeatured Product

Description

High-Sensitivity Colorimetric detection of HSP70

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Application Notes
1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour while shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at at 37°C for 1 hour while shaking at 600rpm. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes while shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

Key Properties

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ReactivityCanine, Human, Monkey, Mouse, Rat
Sample TypesPlasma, Cell Lysates, Tissue
Assay TypeSandwich
Range0.42 - 27 ng/ml
Sensitivity0.038 ng/ml

Storage & Handling

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Storage4°C and -20°C
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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HSP70 1, HSP70 2, HSP70.1, HSP72, HSPA1, HSPA1A, HSPA1B
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Gene Symbol
HSP70 ELISA Kit (High-Sensitivity)

Protocol Information

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96 T
$ 1,110.00
DispatchUsually dispatched within 2-3 weeks
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