HSP90 Antibody (APC)

SKU: orb151257

Description

Rabbit polyclonal to Hsp90 (APC). Hsp90 is a highly conserved and essential stress protein Hsp90 is a highly conserved and essential stress protein...

Images & Validation

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Tested Applications	ELISA, ICC, IF, IHC, IP, WB
Dilution range	WB (1:500), IHC (1:100), ICC/IF (1:100)
Reactivity	Human, Mouse, Rat
Application Notes	A 1:500 dilution of SPC-104 was sufficient for detection of 0.2 mg of purified HSP90 by ECL immunoblot analysis.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Full length protein HSP90
Target	HSP90
Molecular Weight	90kDa
Purification	Protein A Purified
Conjugation	APC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Human Cell line lysates showing detection of HSP90 protein using Rabbit Anti-HSP90 Polyclonal Antibody. Load: 2 μgHsp90beta. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Quick Database Links

Gene Symbol

HSP90

UniProt

P08238

NCBI Gene ID

3326

RefSeq (Protein):NP_031381.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_031381.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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