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HSP90B1 Antibody (N-term)
SKU: orb1930773
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50 |
| Reactivity | Hamster, Human, Mouse |
| Predicted Reactivity | Bovine, Gallus, Monkey, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 92469 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−GRP94, TRA1
Quality Guarantee
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HSP90B1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of HSP90B1 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
HSP90B1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP90B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
HSP90B1 Antibody (N-term) western blot analysis in HepG2, CHO, K562, Hela cell line and mouse liver tissue lysates (35 ug/lane). This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
HSP90B1 Antibody (N-term) (orb1930773)
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