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HSPB8 Antibody
SKU: orb1258139
Featured
Description
Research Area
Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 9
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1). |
| Target | HSPB8 |
| Molecular Weight | Observed: 22kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HSPB8, heat shock 22 kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27 kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, adult, autosomal dominant, CRYAC, PP1629
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Western blot analysis of extracts of various cell lines, using HSPB8 antibody (orb1258139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody (orb1258139) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB8 antibody (orb1258139). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HSPB8
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HSPB8 Antibody (orb1258139)
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