Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit

SKU: orb782197

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2 alpha (8-isoprostane) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2 alpha (8-isoprostane).

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Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-iso-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-iso-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-iso-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	31.25-2000 pg/mL
Sensitivity	8 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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8-isoprostane ELISA Kit, 8-iso-PGF2α ELISA Kit

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Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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