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Human ACE2 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2-His at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1 with a linear range of 1.5-104.5ng/mL. |
| Target | ACE2 |
| Tag | His, Avi tag at the C-terminus |
| Molecular Weight | 86.2 kDa |
| Protein Sequence | Gln18- Ser740 |
| Purity | > 95% by SDS-PAGE. |
Storage & Handling
−| Storage | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human ACE2 Protein, mFc Tag [orb689420]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 109.7 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μgHuman ACE2 Protein, hFc Tag [orb689421]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 109.7 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μgHuman ACE2 Recombinant Protein [orb1231599]
ELISA, WB
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Predicted: 111.2kD Observed: 115-125kD due to glycosylation
HEK293 cells
0.1 mgBiotinylated Human ACE2 Recombinant Protein [orb1231600]
ELISA, WB
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Predicted: 86.5kD Observed: 95-110kD due to glycosylation
HEK293 cells
0.1 mgHuman Recombinant ACE-2 (Fc tag) [orb1736820]
ELISA, WB
92.03 % as determined by Mass Spectrometry.
± 220 kDa
Human
100 μg

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Immobilized human ACE2 recombinant protein at 2μg/mL (100 μL/well) can bind recombinant SARS-CoV-2 (COVID-19) spike S1 with a linear range of 1.5-104.5ng/mL.

Human ACE2 recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 95-110 kD.
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Protocol Information
Human ACE2 Recombinant Protein (orb1231610)
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