Human Angiotensin I Converting Enzyme (ACE) ELISA Kit

SKU: orb774865

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme(ACE).

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Tested Applications

ELISA

Application Notes

standard: 25 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.4-25 ng/mL
Sensitivity	0.17 ng/mL
Concentration	25 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD143 ELISA Kit, ACE1 ELISA Kit, DCP1 ELISA Kit, ACEI ELISA Kit, ACE-I ELISA Kit, Kininase II ELISA Kit, Angiotensin-Converting Enzyme ELISA Kit, Peptidyl-Dipeptidase A ELISA Kit, Dipeptidyl Carboxypeptidase 1 ELISA Kit, Angiotensin-converting enzyme, soluble form ELISA Kit

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Human Angiotensin I Converting Enzyme (ACE) ELISA Kit

Quick Database Links

UniProt

P12821

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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