Human Anti-Growth Hormone Antibody (Anti-GH) ELISA Kit

SKU: orb781684

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Growth Hormone Antibody(Anti-GH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Growth Hormone Antibody(Anti-GH).

Research Area

Metabolism Research, Neuroscience

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	3.13-200 ng/mL
Sensitivity	1.07 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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GH1 ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, hGH-N ELISA Kit, Somatotropin ELISA Kit, Hygetropin ELISA Kit, Jintropin ELISA Kit, Kigtropin ELISA Kit, Pituitary Growth Hormone ELISA Kit, Growth Hormone, Normal ELISA Kit

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Human Anti-Growth Hormone Antibody (Anti-GH) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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